Mrs. Fowler is the mother of Amy Farrah Fowler.
She appears briefly in "The Desperation Emanation" via webcam, where she speaks to Amy and meets Sheldon. Amy introduces him as her boyfriend and tells her that they are having sex regularly. Mrs. Fowler is horrified by how bluntly they describe the frequency of their sex.
As a parent, she made a deal with her daughter to get her to do things. For example, in "The Robotic Manipulation", Amy says she agreed to date someone at least once a year in return for the use of her mother's George Foreman grill. Other words of wisdom from Mrs. Fowler include that playing hooky leads to addictions to reefers and jazz ("The Contractual Obligation Implementation") and that doing a puzzle is like having a thousand friends ("The Scavenger Vortex"). She wouldn't let Amy watch the movie "Grease" while growing up because she thought it would encourage her to join a gang ("The Rothman Disintegration").In "The Bachelor Party Corrosion", Bernadette and Penny get Amy to call her mother to tell her that she broke up with Sheldon. When Amy beats around the bush, Penny grabs the phone and tells her that she just had her ears pierced, baked cookies in the shapes of penises, and broke up with Sheldon. Her mother sends Amy to Penny's closet, the same punishment that she put Amy through as a little girl.
She is played by Annie O'Donnell and listed as "Mrs. Fowler" in the episode's closing credits.
- Annie O'Donnell did the offstage voice of the mother of Dr. Oliver Lorvis in "The Misinterpretation Agitation". She also has worked with another guest star on The Big Bang Theory, Brent Spiner. In 1985-1987, she portrayed the wife of farmer Bob Wheeler in several episodes of Night Court before Brent Spiner was Data on Star Trek: The Next Generation.
- Amy and her mother have an estranged relationship; they don't really get along very well, so they're not very close.
- Amy also has an aunt in Modesto, California an done on Bakersfield, California and a Great-Aunt Flora.